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Thermo Scientific™ Mitochondria Isolation Kit for Tissue

Isolate intact mitochondria from soft and hard tissue samples in about an hour with this versatile, benchtop microcentrifuge-based fractionation kit.

Brand:  Thermo Scientific™ 89801


Product Code. 10521294

  • 403.00 EUR / 1 set

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Description

Description

Isolate intact mitochondria from soft and hard tissue samples in about an hour with Thermo Scientific™ Mitochondria Isolation Kit for Tissue.

The kit provides for two methods for mitochondria isolation. The first method uses a unique reagent-based procedure that enables simultaneous multi-sample processing. The second method relies on traditional Dounce homogenization for tissue disruption and subsequent isolation of the organelle. Both procedures use differential centrifugation to separate the intact mitochondria using a bench-top microcentrifuge and are completed in less than 60 minutes. In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to integrity. The isolated mitochondria may be used for a number of downstream applications, including 1D and 2D Western blotting and protein profiling using mass spectroscopy.

Highlights:

  • Quick and convenient – isolate intact mitochondria in less than 60 minutes

  • Versatile – offers two methods of isolation from both soft- and hard-tissue samples

  • Multi-sample format – reagent-based approach enables simultaneous processing of multiple samples

  • Optional alternate method – reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes

  • Benchtop-compatible – both procedures are performed using a microcentrifuge tube

Includes:

Mitochondria Isolation Reagents A, B and C; bovine serum albumin and a phosphate-buffered saline pack

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Cell Isolation Kit
Liquid
50 Isolations
Upon receipt store kit at 4°C. Product is shipped at ambient temperature.
Organelle Isolation, Subcellular Fractionation
115 mL
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Safety and Handling

Safety and Handling

Product Identifier
  • Mitochondria Isolation Kit for Tissue

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2

Hazard Statement
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information
  • MIXTURE LIST-Contains : Sodium chloride

SDS
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Thermo Scientific™ Mitochondria Isolation Kit for Tissue > Kit; 115mL kit

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For Research Use Only. Not for use in diagnostic procedures.