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Thermo Scientific™ Mitochondria Isolation Kit for Tissue
Isolate intact mitochondria from soft and hard tissue samples in about an hour with this versatile, benchtop microcentrifuge-based fractionation kit.
Brand: Thermo Scientific™ 89801
267.67 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
Isolate intact mitochondria from soft and hard tissue samples in about an hour with Thermo Scientific™ Mitochondria Isolation Kit for Tissue.
The kit provides for two methods for mitochondria isolation. The first method uses a unique reagent-based procedure that enables simultaneous multi-sample processing. The second method relies on traditional Dounce homogenization for tissue disruption and subsequent isolation of the organelle. Both procedures use differential centrifugation to separate the intact mitochondria using a bench-top microcentrifuge and are completed in less than 60 minutes. In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to integrity. The isolated mitochondria may be used for a number of downstream applications, including 1D and 2D Western blotting and protein profiling using mass spectroscopy.Highlights:
Quick and convenient – isolate intact mitochondria in less than 60 minutes
Versatile – offers two methods of isolation from both soft- and hard-tissue samples
Multi-sample format – reagent-based approach enables simultaneous processing of multiple samples
Optional alternate method – reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes
Benchtop-compatible – both procedures are performed using a microcentrifuge tube
Includes:
Mitochondria Isolation Reagents A, B and C; bovine serum albumin and a phosphate-buffered saline pack
Specifications
| Cell Isolation Kit | |
| Liquid | |
| 50 Isolations |
| Upon receipt store kit at 4°C. Product is shipped at ambient temperature. | |
| Organelle Isolation, Subcellular Fractionation | |
| 115 mL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Mitochondria Isolation Kit for Tissue
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Supplemental information
- MIXTURE LIST-Contains : Sodium chloride
For Research Use Only. Not for use in diagnostic procedures.