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Thermo Scientific™ Poly(A) Polymerase, Yeast

Catalyses the addition of adenosine residues onto the 3' ends of RNA. It can be used to add poly(A) tails to RNA in the first step of cloning.

Brand:  Thermo Scientific™ 74225Z25KU

293.37 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Product Code. 15960123

  • 395.00 EUR / Pack of 25

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Description

Description

  • The reaction requires Mn2+ or Mg2+, ATP as substrate, and any RNA containing 3' hydroxyl termini as primer.
  • Substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single 3'-dA residue to the ends of the RNA, a useful technique for labeling RNA at the 3' end.
  • In comparative studies, yeast poly(A) polymerase works more efficiently than E. coli poly(A) polymerase for RNA oligonucleotide-labeling and poly(A) tailing.
  • Poly(A) Polymerase is recommended over T4 RNA Ligase for 3'-end labeling of long RNA molecules.
  • Ribonuclease free
  • 20 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT, 50% glycerol.
  • One unit equals 15 pmol/min AMP incorporated into (riboA)15 at 37°C.
  • 600 units/μL
  • 3' -end labeling of a ribonucleotide with cordycepin-5'-triphosphate.
  • Addition of poly(A) tails to RNA.;2.Labeling the 3' ends of RNA.
  • E. coli strain containing an overproducing clone of Yeast Poly(A) Polymerase.<br
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Shipped on dry ice. Store at -20°C.
Poly(A) RNA Polymerase
25 KU
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Manuals

Brief Protocol: Poly(A) Polymerase, Yeast Version: JAN.2017 File size: 291.3 kB
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Lot Number Certificate Type Date Catalog Number
Lot Number2826514 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number74225Z25KU
Lot Number2807000 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number74225Z25KU
Lot Number2864601 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog Number74225Z25KU
Lot Number2864587 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog Number74225Z25KU
Lot Number2952736 Certificate TypeCertificate of Analysis Date26/01/2025 Catalog Number74225Z25KU
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