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Invitrogen™ ProcartaPlex™ Cell Lysis Buffer
Used to lyse cells under nondenaturing conditions. Invitrogen™ ProcartaPlex™ Cell Lysis Buffer is available in 20mL units that should be stored chilled (for continual use) or frozen (for longer periods of time.) Additional protease inhibitors can be added without any difficulties.
Brand: Invitrogen™ EPX-99999-000
181.48 EUR valid until 2025-02-21
Use promo code "24115" to get your promotional price.
Description
For lysis of adherent cells, 400μL of cell lysis buffer is used for a 10cm dish. For non-adherent cells, 400μL of buffer is used per 107 cells, once they have been washed in PBS and pelleted. Buffer should be chilled and PMSF should be added to the buffer immediately prior to use.
Buffer composition:
- 20mM Tris-HCl (pH 7.5)
- 150mM NaCl
- 1mM Na2EDTA
- 1mM EGTA
- 1% Triton
- 2.5mM sodium pyrophosphate
- 1mM β-glycerophosphate
- 1mM Na3VO4
- 1μg/ml leupeptin
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Specifications
Buffer | |
Canine, Mouse, Rat, Porcine, Non-Human Primate, Human | |
ProcartaPlex™ |
20 mL | |
Luminex™ Instruments |
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Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
429592-000 | Certificate of Analysis | 01/11/2024 | EPX-99999-000 |
409209-000 | Certificate of Analysis | 04/05/2024 | EPX-99999-000 |
388977-000 | Certificate of Analysis | 07/12/2023 | EPX-99999-000 |
382076-000 | Certificate of Analysis | 12/09/2023 | EPX-99999-000 |
360566-000 | Certificate of Analysis | 13/03/2023 | EPX-99999-000 |
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For Research Use Only. Not for use in diagnostic procedures.