Thermo Scientific™ Salmonella Chromogenic Medium

Description

Isolate and differentiate Salmonella species from other Enterobacteriaceae with ready-to-use Thermo Scientific™ Oxoid™ Salmonella Chromogenic Medium. Designed to identify Salmonella species based on their utilization of one chromogenic substrate. The addition of bile salts, novobiocin and cefsulodin inhibit Gram-negative organisms, Proteus and Pseudomonad growth respectively.

Isolate and differentiate Salmonella species from other Enterobacteriaceae with Salmonella Chromogenic Medium.

• Easy to read combination of two chromogens visually differentiates Salmonella from other Enterobacteriaceae.
• Selective for Salmonella due to the additions of bile salts, novobiocin and cefsulodin.
• Ready-to-use convenience of prepared media.

Traditionally, media used to differentiate Salmonella species from other members of the family Enterobacteriaceae depend upon the ability of Salmonella species to produce hydrogen sulphide coupled with their ability to ferment lactose.^{1, 2} These are, however, essentially inadequate methods with a significant number of species not exhibiting these characteristics. Chromogenic media have been developed for rapid and more reliable identification of Salmonella.

Salmonella Chromogenic Medium combines two chromogens for the detection of Salmonella species, 5-Bromo-6-Chloro-3-Indolyl caprylate (Magenta-caprylate) and 5-Bromo-4-Chloro-3-Indolyl b-D galactopyranoside (X-gal). X-gal is a substrate for the enzyme b-D-galactosidase. Most Enterobacteriaceae are b-D-galactosidase positive and produce blue colored colonies on Salmonella Chromogenic Medium. Hydrolysis of the chromogen Mag-caprylate by lactose negative Salmonella species results in magenta colonies which are easily distinguished from the blue colored colonies of other organisms.