missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. DNA Vectors
  5. IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression
  6. 15613788

IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.

Brand:  IBA Lifesciences 5-4433-001

 View more versions of this product

Product Code. 15613788

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products »

Explore more special offers
This item is not returnable. View return policy

Description

Description

pESG-IBA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.

  • NeoR for generation of stable cells lines.
  • Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
  • SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
  • ColE1 + Amp: for propagation in E. coli
  • NeoR: neomycin resistance gene to generate stable cell lines
  • BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Specifications

Liquid
pESG-IBA 33
Neomycin resistance gene
Human CMV Immediate-early
20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
His Tag (6x) for C-terminal
5 μg
Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins
StarGate™ System, Mammalian Host Cells
SV40 large T antigen (e.g. COS1 or COS7)
8
Acceptor Vector for mammalian expression
2°C to 8°C for frequent usage, -20°C for long-term storage
250 ng/μL
Clear
Produktvorschläge

Produktvorschläge

Videos
Sicherheitsdatenblatt (SDS)
Dokumentation Zertifikate
Sonderangebote

Sonderangebote

Berichtigung von Produktinhalten

Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.

Name des Produkts
IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression > StarGate pESG Acceptor Vector: pESG-IBA 33; 6xHistidine-tag for C-terminal; Includes Neomycin resistance gene

Indem Sie auf Absenden klicken, erklären Sie sich damit einverstanden, dass Fisher Scientific sich mit Ihnen in Verbindung setzen kann, um Ihr Feedback in diesem Formular zu bearbeiten. Wir werden Ihre Informationen nicht für andere Zwecke weitergeben. Alle bereitgestellten Kontaktinformationen werden in Übereinstimmung mit unserer Datenschutzrichtlinie aufbewahrt. Datenschutzrichtlinie.

Vielen Dank, dass Sie uns helfen, unsere Website zu verbessern. Ihr Feedback wurde übermittelt