IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression
Description: pESG-IBA144
Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.
Brand: IBA Lifesciences 5-4544-001
Product Code.15594943
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pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.
NeoR for generation of stable cells lines.
Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
ColE1 + Amp: for propagation in E. coli
NeoR: neomycin resistance gene to generate stable cell lines
BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
pESG-IBA mammalian expression vectors
pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.
NeoR for generation of stable cells lines.
Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
ColE1 + Amp: for propagation in E. coli
NeoR: neomycin resistance gene to generate stable cell lines
BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
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