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Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ INVαF' Chemically Competent E. coli
Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector
566.00 EUR - 1000.00 EUR
Specifications
| Bacterial or Yeast Strain | INVαF ́ |
|---|---|
| Cell Type | Chemically Competent E. coli |
| Cloning Method | TA Cloning |
| Content And Storage | This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Format | Kit |
| Product Code | Brand | Bacterial or Yeast Strain | Includes | No. of Reactions | Price | Quantity & Availability | |||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Bacterial or Yeast Strain | Includes | No. of Reactions | Price | Quantity & Availability | |||
|
10003102
|
Invitrogen™
K205001 |
INVαF ́ | Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot INVαF' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. | 20 Reactions |
566.00 EUR
Each |
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|
10740165
|
Invitrogen™
K205040 |
INVαF ́ | Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot INVαF' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. | 40 Reactions |
1000.00 EUR
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
Description
The T7 and Sp6 promoters of the pCRII vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCRII cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
- Fast and convenient: 15-minute, room-temperature ligation
- Efficient: blue/white screening and >80% clones with correct insert
- Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
- Hassle-free: eliminates any enzymatic modifications of PCR product
- Streamlined: does not require use of PCR primers that contain restriction sites
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- T7 and Sp6 promoters for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- M13 forward and reverse primer sites for sequencing
- How TA Cloning Works\
- Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
- Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
- Allows PCR inserts to ligate efficiently with vector
- Kit Configurations
- TA Cloning Kit is offered in a variety of configurations: with One Shot INVF' Chemically Competent E. coli (K2050-01 and K2050-40), One Shot TOP10F' Chemically Competent E. coli (K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20- and 40-reaction kit sizes
Cloning, PCR Cloning
Specifications
| INVαF ́ | |
| TA Cloning | |
| Kit | |
| Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot INVαF' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. | |
| Cloning Kit | |
| pCRII |
| Chemically Competent E. coli | |
| This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
|
| PCR Cloning | |
| One Shot™ | |
| T7, SP6 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- TA Cloning Kit, Dual Promoter, with pCRII Vector and One Shot INV
Signal Word
- Warning
Hazard Category
- Reproductive toxicity Category 2
- Skin sensitiser Category 1
Hazard Statement
- H317-May cause an allergic skin reaction.
- H361-Suspected of damaging fertility or the unborn child.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 2-Mercaptoethanol, Hydrogen chloride
For Research Use Only. Not for use in diagnostic procedures.
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