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Thermo Scientific™ Tango™ CCR5-bla U2OS Cells

Brand: Thermo Scientific™ K1788

Product Code. 10688324

13050.00 EUR / Each

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Description

The Tango™ CCR5-bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ CCR5-bla U2OS cells are functionally validated for Z' and EC₅₀ concentrations of MIP-1a. In addition, Tango™ CCR5-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

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Specifications

Accession Number	NM_000579.1
Cell State	Dividing Cells
Cytoplasmic Tail	Native Tail
For Use With (Application)	FRET
Includes	CCR5-bla U2OS cells, CCR5-bla U2OS Assay Protocol, Certificate of Analysis
Product Line	GeneBLAzer™, Tango™
Readout	End Point
Target Entry	CCR5
Cell Line	U2OS
Druggable Target	GPCR

Assay Entry	Cell-based beta-arrestin recruitment
Content And Storage	LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ CCR5-bla U2OS cells • Tango™ CCR5-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ CCR5-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method	Fluorescence
Gene Symbol	CCR5
Population Status	Single Cell Clone Origin
Quantity	8 x 10⁶ Cells
Reporter Gene	BLA (Beta-Lactamase)
Type	U2OS Cell
System Type	Tango™

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Thermo Scientific™ Tango™ CCR5-bla U2OS Cells

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