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Invitrogen™ TrueCut™ Cas9 Protein v2

A next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency.

Brand: Invitrogen™ A36498

171.08 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

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Product Code. 15739719

198.00 EUR / 100µg

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Description

Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency.

Features include:

Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in difficult targets than the competition
High quality—manufactured under strict ISO 13485 quality standards
Validated protocols for a large number of cell types help you achieve success faster

TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.

Transfection-ready, using either lipid-mediated transfection reagents or electroporation
Eliminates time-consuming cloning steps
Available in 2 concentrations:
- 1 μg/μL for use in standard editing scenarios
- 5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
- For custom sizes or concentrations, please inquire

Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:

Order transfection-ready TrueGuide synthetic gRNAs
Generate transfection-ready gRNA in as little as four hours including template assembly with our GeneArt Precision gRNA Synthesis Kit (Cat. No. A29377)
Have our Custom Services team design, synthesize, and purify in vitro (IVT) gRNA sequences for you

Certifications

CoA

Specifications

Concentration	5 μg/μL
Grade	Molecular Biology
Accession Number	NC_002737.2:854751-858857
Gene ID (Entrez)	901176
Gene Alias	cas5, csn1
Protein Tag	Untagged
Validated Application	Genome Editing
Product Type	Cas9 Protein v2
Product Line	TrueCut™

Components	TrueCut Cas9 Protein v2
Content And Storage	TrueCut Cas9 Protein v2 (5 mg/mL), 100 μg Store at -5°C to -30°C.
Expression System	E. coli
Gene Symbol	Spy_1046
Protein Form	Recombinant
Shipping Condition	Dry Ice
Quantity	100 μg
Research Category	Genome Editing

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Documents

Scientific Resources

Flyers

Flyer: TrueCut Cas9 proteins deliver maximum CRISPR performance File size: 209.2 kB

Product Information

Manuals

User Guide: TrueCut Cas9 Protein Version: D.0 File size: 970.7 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2855228	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog NumberA36498
Lot Number2808473	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA36498
Lot Number2830722	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA36498
Lot Number2844205	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberA36498
Lot Number2891924	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberA36498

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Invitrogen™ TrueCut™ Cas9 Protein v2

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