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ELISA Substrates and Reagents

ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1 – 15 of 1,005 results
1

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
3

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Colorimetric
4

Thermo Scientific™ Stop Solution for TMB Substrates

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

5

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

6

Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

Shipping Condition Ambient
Content And Storage Store at 2°C to 8°C
Form Liquid
Product Type ELISA Stop Solution
Substrate Type HRP, TMB
7

Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use)

This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.

Shipping Condition Room Temperature
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Chemiluminescence
8

BD TMB Substrate Reagent Set

For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.

9

Thermo Scientific™ ELISA Carbonate Coating Buffer

For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA

Promotions are available
10

Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block™ Enhancer

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

11

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

12

Thermo Scientific™ ELISA Blocker Blocking Buffer

Increases signal-to-noise ratio and minimizes background

13

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal™
Detection Method Chemiluminescence
14

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

15

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

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