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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (308)
Real Time PCR Assays and Arrays (302)
Real Time PCR Reagents and Kits (262)

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1 – 15 of 531 results
1
Promotions Available

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

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Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan™
2

TaqMan™ Advanced miRNA cDNA Synthesis Kit

Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.

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3
Promotions Available

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

EDGE
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq DNA Polymerase
Format Tube
Product Type Fast Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Product Line AmpErase™, TaqMan™
4
Promotions Available

Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

EDGE
GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan™
5

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type RNA
Reaction Speed Standard
Product Line AgPath-ID™, Ambion™
6
Promotions Available

Applied Biosystems™ TaqMan™ Multiplex Master Mix

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

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Polymerase AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Multiplex Master Mix
Sample Type DNA
Reaction Speed Fast
Concentration 2X
For Use With (Application) Gene Expression,Virus Detection
PCR Method Multiplex qPCR
Product Line TaqMan™
Detection Method Primer-probe
7
Promotions Available

Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix

TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.

EDGE
Content And Storage Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes:
• Hot-start AmpliTaq Fast DNA Polymerase
• Heat-labile uracil-DNA glycosylase (UNG)
• dNTPs (with dUTP)
• ROX dye (passive reference)
• Optimized buffer

Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple Very High
For Use With (Application) Microbial Detection
PCR Method qPCR
Multiplex Capability Up to 3-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Sensitivity Down to 1 copy
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line TaqPath™
8
Promotions Available

Applied Biosystems™ VetMAX™-Plus qPCR Master Mix

For use with VetMAX™ Reagents and VetMAX™ Controls.

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9

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
GREENER_CHOICE

Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

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10
Promotions Available

Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

TaqMan™ RNase P Detection Reagents Kit

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11
Promotions Available

Applied Biosystems™ MeltDoctor™ HRM Master Mix

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

EDGE
GC-Rich PCR Performance High
Form Liquid
For Use With (Application) Genotyping
PCR Method qPCR
Detection Method HRM
Polymerase AmpliTaq Gold 360 DNA Polymerase
Genotyping Target SNPs (Unknown or Numerous), Epigenetic Targets
Product Type HRM Master Mix
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Label or Dye High Resolution Melting (HRM),SYTO 9
Technique HRM (High Resolution Melting) Analysis, Mutation Scanning
Product Line MeltDoctor™
12
Promotions Available

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

EDGE
Shipping Condition Wet Ice
InhibitorToleranceSimple Medium
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line PowerUP™, SYBR™
13
Promotions Available

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)

TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR.

EDGE
Shipping Condition Dry Ice
InhibitorToleranceSimple High
Concentration 4X
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Thermostability Not Applicable
Passive Reference Dye None
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type RNA, DNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Hot Start Built-In Hot Start
14

Applied Biosystems™ Path-ID™ qPCR Master Mix

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

EDGE
Shipping Condition Dry Ice
Content And Storage 2X qPCR Master Mix is stored at –20°C. Nuclease-free water may be stored at any temperature.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
For Use With (Application) Animal Health,Pathogen Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Hot Start Built-In Hot Start
Product Line Ambion™, Path-ID™
15

Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer

The QuantStudio Absolute Q Isolation Buffer is intended to supplement the materials supplied in the QuantStudio Absolute Q MAP16 Plate Kit.

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