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Thermo Scientific™ 5-IAF (5-iodoacetamido-fluorescein)
5-IAF (5-iodoacetamido-fluorescein)
Brand: Thermo Scientific™ 62246
This item is not returnable.
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Description
Thermo Scientific 5-Iodoacetamido-Fluorescein (5-IAF) is a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. 5-Iodoacetamido-Fluorescein is a sulfhydryl-reactive derivative of fluorescein that labels proteins and other molecules having free thiols (cysteine side chains)Properties of 5-Iodoacetamido-Fluorescein:
- Alternative names: 5-IAF, 5-iodoacetamidofluorescein
- Chemical name: Acetamide, N-(3',6'-dihydroxy-3-oxospiro (isobenzofuran-1(3H), 9'-(9H)xanthen)-5-yl)-2-iodo
- Molecular weight: 515.26±3
- Excitation source: 488 nm spectral line, argon-ion laser
- Excitation wavelength: 494 nm
- Emission wavelength: 518 nm
- Extinction coefficient: > 80,000 M-1cm-1
- CAS #: 63368-54-7
- Solubility: Soluble in DMF; aqueous buffers at pH > 6
- Reactive groups: Iodoacetamide, reacts with sulfhydryls at pH 7.0 to 7.5
Applications: - Label antibodies for use as immunofluorescent probes
- Label oligonucleotides for hybridization probes
- Detect proteins in gels and on Western blots
Specifications
| 5-IAF (5-iodoacetamido-fluorescein), 25mg, Yellow to Orange, Powder, FITC Dye, 515.26±3 Molecular Weight, 494/518nm Excitation/Emission Wavelength, 63368-54-7 CAS Number, –20°C Storage Temperature, Used for Protein Biology, Protein Labeli | |
| Protein labeling and crosslinking, protein biology | |
| 5-IAF (5-iodoacetamido-fluorescein) | |
| >99% by HPLC | |
| FITC (Fluorescein) | |
| 518 |
| Powder | |
| 25 mg | |
| 515.26±3 | |
| -20°C | |
| Yellow to Orange | |
| 494 |
For Research Use Only. Not for use in diagnostic procedures.