Cyclin B1 Monoclonal Antibody (1B10), Invitrogen™

Description

MA5-15706 targets CCNB1 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15706 immunogen is purified recombinant fragment of human CCNB1 expressed in E. Coli. MA5-15706 detects CCNB1 which has a predicted molecular weight of approximately 60kDa.

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.