E-cadherin Monoclonal Antibody (HECD-1), Invitrogen™

Description

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8-U1 mouse myeloma cell line with spleen cells of BALB/c mouse after immunization with human breast tumor cell line MCF-7. Specificity: This antibody specifically reacts with human E-cadherin. This antibody inhibits E-cadherin dependent cell-cell contact. Cross reactivity: This antibody reacts with guinea pig E-cadherin. This antibody does not react with bovine, dog, rat or chicken E-cadherin. Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored. Reconstitution: Dissolve the lyophilized antibody in 50 ?L of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 ?L or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8-U1 mouse myeloma cell line with spleen cells of BALB/c mouse after immunization with human breast tumor cell line MCF-7. Specificity: This antibody specifically reacts with human E-cadherin. This antibody inhibits E-cadherin dependent cell-cell contact. Cross reactivity: This antibody reacts with guinea pig E-cadherin. This antibody does not react with bovine, dog, rat or chicken E-cadherin. Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored. Reconstitution: Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C. Ca2+ should be contained in all the buffers to stabilize cadherin antigen.