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  7. Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
  8. 10612191

Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

Brand:  Thermo Scientific™ EP0441

61.66 EUR valid until 2025-03-21
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Product Code. 10612191

  • 92.50 EUR / 10000 units

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Description

Description

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
60 min.
42°C
RevertAid
Dry Ice
First-Strand cDNA
Reverse Transcription
RNA
Stand-alone Enzyme
Reverse Transcription
10,000 Units
Yes
200 U/μL
Separate components
Up to 13 kb
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Documents

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Product Information

Manuals

User Guide: RevertAid Reverse Transcriptase Version: C.0 File size: 170.6 kB
User Guide: Removal of Genomic DNA from RNA Preparations Version: Jan. 2015 File size: 72.5 kB
User Guide: Guidelines to Avoid RNase Contamination Version: Jan. 2015 File size: 60.2 kB
Certificates

Certificates

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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2821333 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEP0441
Lot Number2871248 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEP0441
Lot Number2871258 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEP0441
Lot Number2893447 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEP0441
Lot Number2935549 Certificate TypeCertificate of Analysis Date26/01/2025 Catalog NumberEP0441
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