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Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
89.50 EUR - 340.00 EUR
Specifications
Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
---|---|
Format | Stand-alone Enzyme |
Reaction Speed | 60 min. |
Technique | Reverse Transcription |
Optimal Reaction Temperature | 42°C |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
10612191
|
Thermo Scientific™
EP0441 |
10,000 Units |
89.50 EUR
10000 units |
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10161310
|
Thermo Scientific™
EP0442 |
5 x 10,000 Units |
340.00 EUR
Pack of 5 |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Specifications
• RevertAid Reverse Transcriptase (200 U/μL) |
|
60 min. | |
42°C | |
Yes | |
200 U/μL | |
Separate components | |
Up to 13 kb |
Stand-alone Enzyme | |
Reverse Transcription | |
RevertAid | |
Dry Ice | |
First-Strand cDNA | |
Reverse Transcription | |
RNA |
For Research Use Only. Not for use in diagnostic procedures.