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Macherey-Nagel™ NucleoSpin™ gDNA Clean-up, Mini kit for DNA clean up and concentration
Concentrate DNA samples more easily and quickly, compared to microdialysis filtrations units. Macherey-Nagel™ NucleoSpin™ gDNA Clean-up Kit cleans and concentrates large-sized genomic DNA from pre-purified DNA from organic extraction and from enzymatic extractions.
Brand: Macherey-Nagel™ 740230.50
This item is not returnable.
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Description
The NucleoSpin™ gDNA Clean-up kit is designed for the rapid purification of previously isolated small and especially high molecular weight genomic DNA. It is used to clean-up and concentrate genomic DNA after crude extraction methods, for example using Trizol, or after enzymatic, or chemical reactions. The time-saving procedures enables a preparation time of just 15 minutes for 10 preps.
- No need for organic denaturants or chloroform extractions
- Any impurities like phenol, enzymes, salts, dyes, labels, nucleotides, small oligonucleotides, and even up to 5% detergents (e.g., SDS, Triton, Tween, Lauroylsarcosin) are removed completely
- Binding Buffer DB and Wash Buffer DW are developed to allow a very gentle binding and washing
- High recovery rates up to 90% for high molecular weight DNA (up to 50 kbp) as well as for small DNA fragments down to 100 bp
- The eluted DNA is ready to use for downstream applications such as PCR, endonuclease restriction, Southern Blotting and labeling
- NucleoSpin™ gDNA Clean-up Columns, Collection Tubes (2 mL), Binding Buffer DB, Wash Buffer DW, Elution Buffer DE
Specifications
| Mini Spin Column | |
| DNA (Genomic) | |
| Elution volume: 50–100μL | |
| NucleoSpin™ gDNA Clean-up Columns (50), 2mL Collection Tubes (50), Binding Buffer DB (25mL), Wash Buffer DW Concentrate (25mL), Elution Buffer DE (13mL) | |
| Nucleo Spin gDNA Clean Up | |
| <150 μL solution containing <25 μg DNA |
| Silica Membrane Technology | |
| Recovery rate: 80–90% | |
| Nucleo Spin gDNA Clean Up | |
| 50 | |
| A260/A280: 1.8–1.9 | |
| <15 min/10 preps |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 2
Hazard Statement
- H225-Highly flammable liquid and vapour.
Supplemental information
- MIXTURE LIST-Contains : ethanol
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Product Title
Macherey-Nagel™ NucleoSpin™ gDNA Clean-up, Mini kit for DNA clean up and concentration > No. of Reactions: 50
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