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  5. Macherey-Nagel™ NucleoSpin™ gDNA Clean-up, Mini kit for DNA clean up and concentration

Macherey-Nagel™ NucleoSpin™ gDNA Clean-up, Mini kit for DNA clean up and concentration

Concentrate DNA samples more easily and quickly, compared to microdialysis filtrations units. Macherey-Nagel™ NucleoSpin™ gDNA Clean-up Kit cleans and concentrates large-sized genomic DNA from pre-purified DNA from organic extraction and from enzymatic extractions.

41.65 EUR - 533.00 EUR

Specifications

Format Mini Spin Column
Isolation Technology Silica Membrane Technology
Sample Type DNA (Genomic)
Efficiency Recovery rate: 80–90%
Elution Volume Elution volume: 50–100μL
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Products 3
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
12773391
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Macherey-Nagel™
740230.250 		250
533.00 EUR
Each
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12763391
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Macherey-Nagel™
740230.10 		10
41.65 EUR
Pack of 10
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12783391
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Macherey-Nagel™
740230.50 		50
138.00 EUR
Each
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Description

Description

The NucleoSpin™ gDNA Clean-up kit is designed for the rapid purification of previously isolated small and especially high molecular weight genomic DNA. It is used to clean-up and concentrate genomic DNA after crude extraction methods, for example using Trizol, or after enzymatic, or chemical reactions. The time-saving procedures enables a preparation time of just 15 minutes for 10 preps.

  • No need for organic denaturants or chloroform extractions
  • Any impurities like phenol, enzymes, salts, dyes, labels, nucleotides, small oligonucleotides, and even up to 5% detergents (e.g., SDS, Triton, Tween, Lauroylsarcosin) are removed completely
  • Binding Buffer DB and Wash Buffer DW are developed to allow a very gentle binding and washing
  • High recovery rates up to 90% for high molecular weight DNA (up to 50 kbp) as well as for small DNA fragments down to 100 bp
  • The eluted DNA is ready to use for downstream applications such as PCR, endonuclease restriction, Southern Blotting and labeling
Kit components:
  • NucleoSpin™ gDNA Clean-up Columns, Collection Tubes (2 mL), Binding Buffer DB, Wash Buffer DW, Elution Buffer DE
Specifications

Specifications

Mini Spin Column
DNA (Genomic)
Elution volume: 50–100μL
Nucleo Spin gDNA Clean Up
<150 μL solution containing <25 μg DNA
Silica Membrane Technology
Recovery rate: 80–90%
Nucleo Spin gDNA Clean Up
A260/A280: 1.8–1.9
<15 min/10 preps
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Safety and Handling

Safety and Handling

Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 2

Hazard Statement
  • H225-Highly flammable liquid and vapour.

Supplemental information
  • MIXTURE LIST-Contains : ethanol

SDS
Documents

Documents

Product Certifications
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