missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Reagents, Antibiotics and Supplements
  5. Additional Cell Culture Reagents and Supplements
  6. Gibco™ rhLaminin-521
  7. 15461945

Gibco™ rhLaminin-521

rhLaminin-521

Brand:  Gibco™ A29248

 View more versions of this product

Product Code. 15461945

  • 86.25 EUR / 100µg

Please to purchase this item. Need a web account? Register with us today!

Edge
Explore more special offers
This item is not returnable. View return policy

Description

Description

Recombinant human Laminin 521 (rhLaminin-521) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium, rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 also provides optimum reprogramming efficiency of human somatic cells as well as human pluripotent stem cell survival during feeder-dependent to feeder-free transitions when used in conjunction with Essential 8 Medium. For a kit that combines the medium and substrate, see the Essential 8™ Adaptation Kit.

rhLaminin-521 is:
  • Robust—transition cells between feeder-dependent and feeder-free culture systems with minimal effort
  • Efficient—easily reprogram human fibroblasts to pluripotent stem cells
  • Physiologically relevant—expand cells with minimal differentiation
  • Scalable—passage stem cell lines as single cells with no impact to genetic stability

    Trouble-free transition
    When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.

    Efficient reprogramming
    rhLaminin-521 has been shown to increase the number of colonies obtained during reprogramming of human fibroblasts, which can be important for in vitro disease modeling.

    Confident expansion
    rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.

    Easy scale-up
    rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.
    TRUSTED_SUSTAINABILITY
    • Specifications

    Specifications

    Store in freezer (-5 to -20°C) and protect from light.
    Xeno-free
    Extracellular Matrix
    hESC-Qualified
    Dry Ice
    Stem Cells
    Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
    6 to 8
    100μg
    Xeno-Free
    Product Suggestions

    Product Suggestions

    Videos
    SDS
    Documents

    Documents

    Certificates
    Special Offers

    Special Offers

    Product Content Correction

    Your input is important to us. Please complete this form to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

    For Research Use Only. Not for use in diagnostic procedures.