CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. BD Horizon™ BV480 Mouse Anti-Human CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε